It will be the first time the bitter rivals have met at the SCG in the opening round for 92 years, with the showdown entrenched in decades of rancorous history and loathing between the sides.
The grudge match will also see former Souths star forward Angus Crichton playing his first game for the Roosters, with tensions sure to be high all match.
The Roosters and the Rabbitohs have the fiercest rivalry in NRL history, extending back to the foundation of both clubs in 1908 when the New South Wales Rugby League began.
The full Round 1 fixture can be found below, as revealed by The Daily Telegraph. The full season draw is to set to be announced this Thursday.
ROUND 1 2019 NRL DRAW
THURSDAY, MARCH 14
STORM v BRONCOS
7.40pm at AAMI Park (Fox Sports and Channel 9)
FRIDAY, MARCH 15
KNIGHTS v SHARKS
6pm at McDonald Jones Stadium (Fox Sports)
ROOSTERS v RABBITOHS
7.40pm at SCG (Fox Sports and Channel 9)
SATURDAY, MARCH 16
WARRIORS v BULLDOGS
4pm at Mt Smart Stadium (Fox Sports)
TIGERS v SEA EAGLES
6pm at Campbelltown Stadium/Leichhardt Oval (Fox Sports)
COWBOYS v DRAGONS
8pm at 1300Smiles Stadium (Fox Sports)
SUNDAY, MARCH 17
PANTHERS v EELS
4pm at Penrith Stadium (Fox Sports and Channel 9)
TITANS v RAIDERS
6.30pm at Cbus Super Stadium (Fox Sports)