Tupo failed to finish the match against the Bulldogs with a suspected pec tear.
Scans over the weekend have since confirmed the extent of the injury, with Tupou expected to be sidelined for 10-12 weeks while he recovers.
The Roosters take on the Knights in Round 3 and a replacement for Tupou will be named tomorrow.
Confirmation today Daniel Tupou suffered a torn pectoral muscle on the weekend & will require a 10-12 week recovery. Will likely undergo surgery to repair tear. Examples of NRL players with torn pecs & their return to play times below. Shows how treatment has improved over time pic.twitter.com/autHVTforz
— NRL PHYSIO (@nrlphysio) March 19, 2018