Maori All Stars centre Joseph Manu has been ruled out of the cultural clash after suffering a minor facial fracture at training, while Jared Warea-Hargreaves will miss out following a flare up.
The Roosters were training in Queenstown during the club's annual pre-season trip across the ditch when the outside back suffered a minor fracture to his face during a training drill.
It isn't the first time Manu has suffered a facial injury, having fractured his cheekbone in the Latrell Mitchell incident back in late 2021.
Warea-Hargreaves suffered a flare up of a lower back injury as well as his hamstring, causing the fiery prop to withdraw from what could potentially have been his final All Stars match.
Carrying a recovery period of roughly a month, Manu will be racing the clock to prove his fitness for their Round 1 clash against the Dolphins, while 'JWH' is expected to be for the season opener.
Elsewhere, Ezra Mam and Chris Smith have joined Josh Addo-Carr and Will Smith in removing themselves from the match, with Daine Laurie, Isaiah Tass, Braydon Trindall and Albert Kelly called into the squad.
The All Stars clash is just a week out, with the two cultures set to clash at Rotorua International Stadium next Saturday.