A statement from the club confirmed his return would be put on hold, with the club captain now to have scans which will determine if he requires surgery.
"Croker was set to make his return to the field this weekend after injuring his shoulder against the Bulldogs in round 9.
"He will have scans in the coming days to determine the extent of the injury and see if it requires surgery."
Croker, who had career-saving stem cell surgery on his knee during the off-season, has played just a single game in the NRL this season against the Bulldogs in Round 9.
After a strong performance on his return, Croker injured his shoulder in the final minutes of the game as he raced to stop a potential Matt Dufty try.
The injury put him back on the sidelines for a fortnight, however, the new injury could place into question his ability to return this season, pending on the results of the scan and whether surgery is needed.
According to The Canberra Times, Croker sustained the injury on Tuesday night after teams had been named, rather than at training as had been first thought.
Croker, who has 292 NRL games, was looking for a run in the team to approach 300 games, however, that progress will now be denied.
It's tipped Sebastian Kris, who is on the bench, will come into the starting side, while one of Harry Rushton, Corey Harawira-Naera or Matt Frawley will come onto the bench, pending on the balance coach Ricky Stuart wants to carry into the game.